Journal: Biologically-Inspired Systems

Article Title: Combined Discrete and Continual Approaches in Biological Modelling

doi: 10.1007/978-3-030-41528-0

Figure Lengend Snippet: Fig. 6.8 (a) Scheme of the experimental setup and (b) the force–distance diagram of the force measurement. The feather was separated at the interface between two neighboring barbs using a micromanipulator equipped with a force transducer attached to the vane. This separation caused the detachment of hook barbules (HB) from bow barbules (BB). The arrow in (a) indicates the direction of pulling. After some final degree of displacement was reached, the force transducer was returned to its initial position (healing). (From Kovalev et al. 2014)

Article Snippet: For relaxation, the specimen was placed in a phosphate buffer solution (pH 7.0) 2 h before the force measurements, which were carried out with a load cell force transducer (FORT100; World Precision Instruments, Sarasota, FL, USA).

Techniques:

Journal: Biologically-Inspired Systems

Article Title: Combined Discrete and Continual Approaches in Biological Modelling

doi: 10.1007/978-3-030-41528-0

Figure Lengend Snippet: Fig. 7.11 (a) Section through the wing hinge of L. migratoria. After the standard fixation and dehydration procedure (Gorb 2004), the wing hinges were embedded in Technovit 7100 glycol methacrylate; 3.5 μm thick sections were prepared and stained with toluidine blue. (b) Experimental setup for force measurements on a native resilin patch from the wing hinge ligament with the arrow indicating the loading direction. Abbreviations: pt plate, rl resilin with chitin lamellae, rs resilin sample, sb sapphire sphere, sc solid cuticle, sn force transducer, t transition zone, wr stainless steel wire. (From Kovalev et al. 2018)

Article Snippet: For relaxation, the specimen was placed in a phosphate buffer solution (pH 7.0) 2 h before the force measurements, which were carried out with a load cell force transducer (FORT100; World Precision Instruments, Sarasota, FL, USA).

Techniques: Staining

Journal: Molecular Vision

Article Title: Technical brief: Direct, real-time electrochemical measurement of nitric oxide in ex vivo cultured human corneoscleral segments

doi:

Figure Lengend Snippet: Experimental setup for the study. A: Workflow for electrochemical nitric oxide (NO) measurement using NO-sensing electrode. Cultured human donor corneoscleral segments (C = cornea; S = sclera; TM = trabecular meshwork) were washed 3X with PBS and placed in a 24-well plate with 1 ml of PBS. Drug treatments were performed after a stable baseline was achieved. The output current corresponds to the level of NO generated (~10 pA/nM), detected using a free radical analyzer (TBR1025). The signal is amplified by a four-channel Lab-Trax amplifier and analyzed using LabScribe3 software. B: Workflow for fluorochemical measurement of NO using DAF-FM assay (intracellular NO-binding dye). Quadrants of the human donor corneoscleral segment were cultured at 37 °C in DMEM media supplemented with 2% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS) and treated with 10 μM NO-binding fluorescent DAF-FM dye for 30 min. Cells and tissues were washed 1X with PBS and treated with different drugs or vehicle and incubated at 37 °C for an additional 30 min. Tissues were then washed 3X with PBS, and the trabecular meshwork (TM) rim was removed and imaged using fluorescence microscopy.

Article Snippet: Direct measurements of NO were performed using a TBR1025 free radical analyzer and NO-sensing electrode (ISO-NOPF200) obtained from World Precision Instruments (WPI; Sarasota, FL).

Techniques: Cell Culture, Generated, Amplification, Software, Binding Assay, Incubation, Fluorescence, Microscopy

Journal: Molecular Vision

Article Title: Technical brief: Direct, real-time electrochemical measurement of nitric oxide in ex vivo cultured human corneoscleral segments

doi:

Figure Lengend Snippet: Troubleshooting solutions for real-time electrochemical measurement of NO in human corneoscleral segments (adapted from World Precision Instruments).

Article Snippet: Direct measurements of NO were performed using a TBR1025 free radical analyzer and NO-sensing electrode (ISO-NOPF200) obtained from World Precision Instruments (WPI; Sarasota, FL).

Techniques: Transferring